Part:BBa_K590063:Design

mamHIEJKL_pLacGFP_pGA3K3

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 12370
  Illegal PstI site found at 5183
  Illegal PstI site found at 8860
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 12370
  Illegal NheI site found at 11025
  Illegal PstI site found at 5183
  Illegal PstI site found at 8860
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 12370
  Illegal BglII site found at 8564
  Illegal BglII site found at 12385
  Illegal BamHI site found at 9642
  Illegal XhoI site found at 89
  Illegal XhoI site found at 6383
  Illegal XhoI site found at 9657
  Illegal XhoI site found at 9818
  Illegal XhoI site found at 10661
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 12370
  Illegal PstI site found at 5183
  Illegal PstI site found at 8860
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 12370
  Illegal PstI site found at 5183
  Illegal PstI site found at 8860
  Illegal NgoMIV site found at 238
  Illegal NgoMIV site found at 1254
  Illegal NgoMIV site found at 2589
  Illegal NgoMIV site found at 3920
  Illegal NgoMIV site found at 6770
  Illegal NgoMIV site found at 8586
  Illegal NgoMIV site found at 8953
  Illegal AgeI site found at 5857
  Illegal AgeI site found at 6272
  Illegal AgeI site found at 11111
  Illegal AgeI site found at 11434
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 651
  Illegal BsaI site found at 1986
  Illegal BsaI site found at 3317
  Illegal BsaI.rc site found at 6963
  Illegal BsaI.rc site found at 12139
  Illegal SapI site found at 825
  Illegal SapI site found at 2160
  Illegal SapI site found at 3491
  Illegal SapI.rc site found at 9232
  Illegal SapI.rc site found at 9553
  

Design Notes

TBD

Source

Made From Gibson reaction